LOCUS       sequence-52771-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTPRO
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagagganc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatgaa cccggttcaa ctggatgact ttgatgccta
      241 tattaaggat atggccaaag actctgacta taaattttct cttcagtttg aggagttgaa
      301 attgattgga ctggatatcc cacactttgc tgcagatctt ccactgaatc gatgtaaaaa
      361 ccgttacaca aacatcctac catatgactt cagccgtgtg agattagtct ccatgaatga
      421 agaggaaggt gcagactaca tcaatgccaa ctatattcct ggatacaact caccccagga
      481 gtatattgcc acccaggggc cactgcctga aaccagaaat gacttctgga agatggtcct
      541 gcaacaaaag tctcagatta ttgtcatgct cactcagtgt aatgagaaaa ggagggtgaa
      601 atgtgaccat tactggccat tcacggaaga acctatagcc tatggagaca tcactgtgga
      661 gatgatttca gaggaagagc aggacgactg ggcctgtaga cacttccgga tcaactatgc
      721 tgacgagatg caggatgtga tgcattttaa ctacactgca tggcctgatc atggtgtgcc
      781 cacagcaaat gctgcagaaa gtatcctgca gtttgtacac atggtccgac agcaagctac
      841 caagagcaaa g
//
