LOCUS       sequence-52772-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HAO1
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             183..203
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg caccatcatc atcatcattc ttctggtgta gatctgggta
      181 ccgagaacct gtacttccaa tccatgctcc cccggctaat ttgtatcaat gattatgaac
      241 aacatgctaa atcagtactt ccaaagtcta tatatgacta ttacaggtct ggggcaaatg
      301 atgaagaaac tttggctgat aatattgcag cattttccag atggaagctg tatccaagga
      361 tgctccggaa tgttgctgaa acagatctgt cgacttctgt tttaggacag agggtcagca
      421 tgccaatatg tgtgggggct acggccatgc agcgcatggc tcatgtggac ggcgagcttg
      481 ccactgtgag agcctgtcag tccctgggaa cgggcatgat gttgagttcc tgggccacct
      541 cctcaattga agaagtggcg gaagctggtc ctgaggcact tcgttggctg caactgtata
      601 tctacaagga ccgagaagtc accaagaagc tagtgcggca ggcagagaag atgggctaca
      661 aggccatatt tgtgacagtg gacacacctt acctgggcaa ccgtctggat gatgtgcgta
      721 acagattcaa actgccgcca caactcagga tgaaaaattt tgaaaccagt actttatcat
      781 tttctcctga ggaaaatttt ggagacgaca gtggacttgc tgcatatgtg gctaaagcaa
      841 tagacccatc tatcagctgg gaagatatca aatggctgag aagactgaca tcattgccaa
      901 ttgttgcaaa gggcattttg agaggtgatg atgcc
//
