LOCUS       sequence-52773-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CAMK2A
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..204
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt agatctgggt
      181 accgagaacc tgtacttcca atccatgtac cagctcttcg aggaattggg caagggagcc
      241 ttctcggtgg tgcgaaggtg tgtgaaggtg ctggctggcc aggagtatgc tgccaagatc
      301 atcaacacaa agaagctgtc agccagagac catcagaagc tggagcgtga agcccgcatc
      361 tgccgcctgc tgaagcaccc caacatcgtc cgactacatg acagcatctc agaggaggga
      421 caccactacc tgatcttcga cctggtcact ggtggggaac tgtttgaaga tatcgtggcc
      481 cgggagtatt acagtgaggc ggatgccagt cactgtatcc agcagatcct ggaggctgtg
      541 ctgcactgcc accagatggg ggtggtgcac cgggacctga agcctgagaa tctgttgctg
      601 gcctccaagc tcaagggtgc cgcagtgaag ctggcagact ttggcctggc catagaggtg
      661 gagggggagc agcaggcatg gtttgggttt gcagggactc ctggatatct ctccccagaa
      721 gtgctgcgga aggacccgta cgggaagcct gtggacctgt gggcttgtgg ggtcatcctg
      781 tacatcctgc tggttgggta ccccccgttc tgggatgagg accagcaccg cctgtaccag
      841 cagatcaaag ccggcgccta tgatttccca tcgccggaat gggacactgt caccccggaa
      901 g
//
