LOCUS       sequence-52774-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG584
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             298..318
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             331..375
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(377..401)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aacttggact tactaactaa cggattatat ttaaattttc agaacattaa catggcacta
       61 ctactagaag gtggaggtca ctacctttgc gattttaaaa ctacttacaa agcaaagaag
      121 gtcgtccaac ttccagatgc acactttgtt gatcacagaa ttgaaatact aggaaacgat
      181 tcggattaca acaaagttaa gctatacgaa cacgcagttg caagatacag tcctctacca
      241 agtcaagcat ggtgcccggg ggatcggtgg agctccaccg gtggcggccg ctcgagagag
      301 aatctttatt ttcagggcgc cgccaaattc aaagaaaccg ctgctgctaa attcgaacgc
      361 cagcacatgg acagccaccc agctttcttg tacaaagtgg agatgaaagc ggcatcaaat
      421 agtgtctcgt cggccggagg atcagtgtca cctacgacaa cccagccacc actaccacca
      481 gggcagtcgt cacatcctca aatctacgat caacaggtat tcacaatcaa caaatgttga
      541 taatattaca gttatatttt tcagatgcaa tattacttcg ctgccgcgat gccaaatcag
      601 ccgatggcaa catacgcggc gcagaacgga tcttctcagc aatatgcgcc agctgcccca
      661 tactatcagg atgcgaatgg acaatatgtt caagttcctg ccaacggttc tatggctccc
      721 cagcaacata tgatggtgtc aggacagccg tacctttata tggctcagcc acagcaaggt
      781 gcgcaacaag tgatgcaatc gggacagcct caacttattt actatca
//
