LOCUS       sequence-52778-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG593
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             311..331
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             344..388
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(390..414)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggagtaagt ttaaacttgg acttactaac taacggatta tatttaaatt ttcagaacat
       61 taacatggca ctactactag aaggtggagg tcactacctt tgcgatttta aaactactta
      121 caaagcaaag aaggtcgtcc aacttccaga tgcacacttt gttgatcaca gaattgaaat
      181 actaggaaac gattcggatt acaacaaagt taagctatac gaacacgcag ttgcaagata
      241 cagtcctcta ccaagtcaag catggtgccc gggggatcgg tggagctcca ccggtggcgg
      301 ccgctcgaga gagaatcttt attttcaggg cgccgccaaa ttcaaagaaa ccgctgctgc
      361 taaattcgaa cgccagcaca tggacagcca cccagctttc ttgtacaaag tggagatgaa
      421 agcggcatca aatagtgtct cgtcggccgg aggatcagtg tcacctacga caacccagcc
      481 accactacca ccagggcagt cgtcacatcc tcaaatctac gatcaacagg tattcacaat
      541 caacaaatgt tgataatatt acagttatat ttttcagatg caatattact tcgctgccgc
      601 gatgccaaat cagccgatgg caacatacgc ggcgcagaac ggatcttctc agcaatatgc
      661 gccagctgcc ccatactatc aggatgcgaa tggacaatat gttcaagttc ctgccaacgg
      721 ttctatggct ccccagcaac atatgatggt gtcaggacag ccgtaccttt atatggctca
      781 gccacagcaa ggtgcgcaac aagtgatgca atcgggacag c
//
