LOCUS       sequence-52780-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   DasGupta R, Kaykas A, Moon RT, Perrimon N
  TITLE     Functional genomic analysis of the Wnt-wingless signaling pathway.
  JOURNAL   Science. 2005 May 6;308(5723):826-33. doi: 10.1126/science.1109374. 
            Epub 2005 Apr 7.
  PUBMED    15817814
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1109374"; journalName: "Science"; date: "2005-05-6-
            6"; volume: "308"; issue: "5723"; pages: "826-33"
COMMENT     Sequence Label: M72 Super(16X)TOPFLASH
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(94..125)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    358..449
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(390..409)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    463..511
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta ccggaaatgc
       61 caagcttttt accaacagta ccggaatgcc aagctggaat tcgagcttcc attatatacc
      121 ctctagagtc tagatctcga gcccgggcta gcacgcgggc gcgacgcgtc ctttgatctt
      181 accccctttg atcttacccc ctttgatctt accccctttg atctcgcgcc cctttgatct
      241 tacccccttt gatcttaccc cctttgatct tacccccttt gatctcgcgc ccctttgatc
      301 ttaccccctt tgatcttacc ccctttgatc tcgcgtaaga gctcggtacc tatcgataga
      361 gaaatgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt
      421 tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac
      481 acagatgtaa tgaaaataaa gatattttat tgcggccgct ccaagtacct cccgtacctt
      541 aatattactt acttatcatg gtagcttggg ctggcgtaat agcgaagagg cccgcaccga
      601 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat
      661 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
      721 aatcggcaaa atcccttata aaatcaaaag aatagaccga gatagggttg agtgttgttc
//
