LOCUS       sequence-52782-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             182..202
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             554..571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(626..644)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     primer_bind     complement(813..832)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag atctgggtac
      181 cgagaacctg tacttccaat ccatgcctca atgtaagtct attacactag agcgaggacc
      241 agatggctta ggcttcagta tagttggagg atatggcagc cctcatggag acttacccat
      301 ttatgttaaa acagtgtttg caaagggagc agcctctgaa gacggacgtc tgaaaagggg
      361 cgatcagatc attgctgtca atgggcagag tctagaagga gtcacccatg aagaagctgt
      421 tgccatcctt aaacggacaa aaggcactgt cactttgatg gttctctcta gtgatgaaac
      481 ttctgtttga cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg
      541 gccgcacttc gagcaccacc accaccacca ctgagaatcc ggctgcctaa caaagcccga
      601 aaggaagctg anttggctgc tgccaccgct gagcaataac tagcataacc ccttgggggc
      661 ctctaaacgg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      721 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      781 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      841 cgca
//
