LOCUS       sequence-52783-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    42..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             81..103
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             114..131
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             156..176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             536..553
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(606..624)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      620..667
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(791..810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
       61 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgcaccatc
      121 atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgg
      181 gattaagaac agtcgaaatg aaaaagggcc ctactgactc actgggaatc agcatcgctg
      241 gaggagtagg cagcccactt ggtgatgtgc ctatatttat tgcaatgatg cacccaactg
      301 gagttgcagc acagacccaa aaactcagag ttggggatag gattgtcacc atctgtggca
      361 catccactga gggcatgact cacacccaag cagttaacct actgaaaaat gcatctggct
      421 ccattgaaat gcaggtggtt gctggaggag acgtgagtga aacttctgtt tgacagtaaa
      481 ggtggatacg gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca
      541 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      601 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      661 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      721 gcattaagcg cggcgggtgt ggtggntacg cgcagcgtga ccgctacact tgccagcgcc
      781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggcttttcc
      841 ccgtcaagct ctaaatcggg g
//
