LOCUS       sequence-52784-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    65..89
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             104..126
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..199
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             553..570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(623..641)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      637..684
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(809..828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta
       61 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
      121 aggagatata catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga
      181 gaacctgtac ttccaatcca tgagtgagac atttgatgta gaactcacta aaaatgtcca
      241 aggattagga attaccattg ctggctacat tggagataaa aaattggaac cttcaggaat
      301 ctttgtaaag agcattacaa aaagcagtgc cgttgagcat gatggaagaa tccaaattgg
      361 agaccaaatt atagcagtag atggcacaaa ccttcagggt tttactaatc agcaagcagt
      421 agaggtattg cgacatacag gacaaactgt gctcctgaca ctaatgagga gaggagaaac
      481 ttctgtttga cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg
      541 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      601 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      661 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacngnn cagcgtgacc
      781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      901 agtgct
//
