LOCUS       sequence-52785-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MPDZ
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             139..156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             615..632
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(685..703)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      699..746
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga tctgggtacc
      181 gagaacctgt acttccaatc catgcctgct tgtgatgaat ttgatcagct tatcaaaaat
      241 atggcccagg gtcgccatgt agaagttttt gagctcctca aacctccatc tggaggcctt
      301 gggtttagtg ttgtgggact aagaagtgaa aacagaggag agctgggaat atttgttcaa
      361 gagatacaag agggcagtgt ggcccataga gatggaagat tgaaagaaac tgatcaaatt
      421 cttgctatca atggacaggc tcttgatcag acaattacac atcagcaggc tatcagcatc
      481 ctgcagaaag ccaaagatac tgtccagcta gttattgcca gaggctcatt gcctcagtat
      541 tacaaggttt gacagtaaag gtggatacgg atccgaattc gagctccgtc gacaagcttg
      601 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      661 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      721 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccnga ttggcgaatg
      781 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      841 cgctacactt gccagcgccc tagcgcccgc tcctt
//
