LOCUS       sequence-52789-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CREBBP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..204
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             624..641
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(694..712)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      708..755
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(879..898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt agatctgggt
      181 accgagaacc tgtacttcca atccatgcgc aaaaaaattt ttaaaccgga agaactgcgt
      241 caggcgctga tgccgaccct ggaagcgctg tatcgccagg atccggaaag cctgccgttt
      301 cgtcagccgg tggatccgca gctgctgggt atcccggatt attttgatat tgtgaaaaac
      361 ccgatggatc tgagcaccat caaacgcaaa ctggataccg gccagtatca ggaaccgtgg
      421 cagtatgtgg atgatgtttg gctgatgttt aataatgcgt ggctgtataa ccgtaaaacc
      481 agccgtgtgt ataaattctg tagcaaactg gcggaagttt ttgaacagga aattgatccg
      541 gtgatgcaga gcctgggctg acagtaaagg tggatacgga tccgaattcg agctccgtcg
      601 acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      661 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      721 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      781 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      901 ctttctcgcc acgttcgccg gctttccccg tca
//
