LOCUS       sequence-52790-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FH
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             182..202
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag atctgggtac
      181 cgagaacctg tacttccaat ccatgatggc aagccaaaat tccttccgga tagaatatga
      241 tacctttggt gaactaaagg tgccaaatga taagtattat ggcgcccaga ccgtgagatc
      301 tacgatgaac tttaagattg gaggtgtgac agaacgcatg ccaaccccag ttattaaagc
      361 ttttggcatc ttgaagcgag cggccgctga agtaaaccag gattatggtc ttgatccaaa
      421 gattgctaat gcaataatga aggcagcaga tgaggtagct gaaggtaaat taaatgatca
      481 ttttcctctc gtggtatggc agactggatc aggaactcag acaaatatga atgtaaatga
      541 agtcattagc aatagagcaa ttgaaatgtt aggaggtgaa cttggcagca agatacctgt
      601 gcatcccaac gatcatgtta ataaaagcca gagctcaaat gatacttttc ccacagcaat
      661 gcacattgct gctgcaatag aagttcatga agtactgtta ccaggactac agaagttaca
      721 tgatgctctt gatgcaaaat ccaaagagtt tgcacagatc atcaagattg gacgtactca
      781 tactcaggat gctgttccac ttactcttgg gcaggaattt agtggttatg ttcaacaagt
      841 aaaatatgca atgacaagaa taaaagctgc catgccaaga atctatgagc tcgcagctgg
      901 aggcactgct
//
