LOCUS       sequence-52794-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CA13
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..204
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt agatctgggt
      181 accgagaacc tgtacttcca atccatgtcg aggctcagct ggggataccg cgagcacaac
      241 ggtcctattc actggaagga atttttccct attgctgatg gtgatcagca atctccaatt
      301 gagattaaaa ccaaagaagt gaaatatgac tcttccctcc gaccacttag tatcaagtat
      361 gacccaagct cagctaaaat catcagcaac agcggccatt ccttcaatgt tgactttgat
      421 gacacagaga acaaatcagt tctgcgtggt ggtcctctca ctggaagcta caggttacgg
      481 caggttcacc ttcactgggg gtccgctgat gaccacggct ccgagcacat agtagatgga
      541 gtgagctatg ctgcagagct ccatgttgtt cactggaatt cagacaaata ccccagcttt
      601 gttgaggcag ctcatgaacc agatggactg gctgtcttgg gagtgttttt acagattggt
      661 gaacctaatt cccaactgca aaagattact gacactttgg attccattaa agaaaagggt
      721 aaacaaactc gattcacaaa ttttgaccta ttgtctctgc ttccaccatc ctgggactac
      781 t
//
