LOCUS       sequence-52795-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CSNK1G1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatgag ggttggcaag aagataggat gtgggaactt
      241 cggagagctc agattaggta aaaatctcta caccaatgaa tatgtagcaa tcaaactgga
      301 accaataaaa tcacgtgctc cacagcttca tttagagtac agattttata aacagcttgg
      361 cagtgcaggt gaaggtctcc cacaggtgta ttactttgga ccatgtggga aatataatgc
      421 catggtgctg gagctccttg gccctagctt ggaggacttg tttgacctct gtgaccgaac
      481 atttactttg aagacggtgt taatgatagc catccagctg ctttctcgaa tggaatacgt
      541 gcactcaaag aacctcattt accgagatgt caagccagag aacttcctga ttggtcgaca
      601 aggcaataag aaagagcatg ttatacacat tatagacttt ggactggcca aggaatacat
      661 tgaccccgaa accaaaaaac acatacctta tagggaacac aaaagtttaa ctggaactgc
      721 aagatatatg tctatcaaca cgcatcttgg caaagagcaa agccggagag atgatttgg
//
