LOCUS       sequence-52798-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: VRK3
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             194..214
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt
      181 agatctgggt accgagaacc tgtacttcca atccatgacc acctcacttg aagctttgcc
      241 cacagggaca gtgctgacag acaagagtgg gcgacagtgg aagctgaagt ccttccagac
      301 cagggacaac cagggcattc tctatgaagc tgcacccacc tccaccctca cctgtgactc
      361 aggaccacag aagcaaaagt tctcactcaa actggatgcc aaggatgggc gcttgttcaa
      421 tgagcagaac ttcttccagc gggccgccaa gcctctgcaa gtcaacaagt ggaagaagct
      481 gtactcgacc ccactgctgg ccatccctac ctgcatgggt ttcggtgttc accaggacaa
      541 atacaggttc ttggtgttac ccagcctggg gaggagcctt cagtcggccc tggatgtcag
      601 cccaaagcat gtgctgtcag agaggtctgt gctgcaggtg gcctgccggc tgctggatgc
      661 cctggagttc ctccatgaga atgagtatgt tcatggaaat gtgacagctg aaaatatctt
      721 tgtggatcca gaggaccaga gtcaggtgac tttggcaggc tatggcttcg ccttccgcta
      781 ttgcccaagt ggcaaacacg tggcctacgt ggaaggcagc aggagccctc acgaggggga
      841 ccttgagttc attagcatgg acctgcacaa gggatgcggg ccctcccgc
//
