LOCUS       sequence-52801-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTPN7
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             116..138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             191..211
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga
      181 tctgggtacc gagaacctgt acttccaatc catgaacaca ccccgggagg tcaccctaca
      241 ctttctgcgc actgctggac acccccttac ccgctgggcc cttcagcgcc agccacccag
      301 ccccaagcaa ctggaagaag aattcttgaa gatcccttca aactttgtca gccccgaaga
      361 cctggacatc cctggccacg cctccaagga ccgatacaag accatcttgc caaatcccca
      421 gagccgtgtc tgtctaggcc gggcacagag ccaggaggac ggagattaca tcaatgccaa
      481 ctacatccga ggctatgacg ggaaggagaa ggtctacatt gccacccagg gccccatgcc
      541 caacactgtg tcggacttct gggagatggt gtggcaagag gaagtgtccc tcattgtcat
      601 gctcactcag ctccgagagg gcaaggagaa atgtgtccac tactggccca cagaagagga
      661 aacctatgga cccttccaga tccgcatcca ggacatgaaa gagtgcccag aatacactgt
      721 gcggcagctc accatccagt accaggaaga gcgccggtca gtaaagcaca tcctcttttc
      781 ggcctggcca gaccatcaga caccagaatc agctgggccc ctgctgcgcc tagtggcaga
      841 ggtggaggag agcccggaga cagccgccca ccccgggcct atcg
//
