LOCUS       sequence-52802-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLYR1
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    83..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             122..144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             197..217
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      121 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
      181 tgtagatctg ggtaccgaga acctgtactt ccaatccatg ggcagcatca cacccacaga
      241 caaaaagata ggatttttgg gccttggtct catgggaagt ggaatcgtct ccaacttgct
      301 aaaaatgggt cacacagtga ctgtctggaa ccgcactgca gagaaatgtg atttgttcat
      361 ccaggagggg gcccgtctgg gaagaacccc cgctgaagtc gtctcaacct gcgacatcac
      421 tttcgcctgc gtgtcggatc ccaaggcggc caaggacctg gtgctgggcc ccagtggtgt
      481 gctgcaaggg atccgccctg ggaagtgcta cgtggacatg tcaacagtgg acgctgacac
      541 cgtcactgag ctggcccagg tgattgtgtc cagggggggg cgctttctgg aagcccccgt
      601 ctcagggaat cagcagctgt ctaatgacgg gatgttggtg atcttagcgg ctggagacag
      661 gggcttatat gaggactgca gcagctgctt ccaggcgatg gggaagacct ccttcttcct
      721 aggtgaagtg ggcaatgcag ccaagatgat gctgatcgtg aacatggtcc aagggagctt
      781 catggccact attgccgagg ggctgaccct ggcccaggtg acaggccagt cccagcagac
      841 actcttggac atcctcaatc agggacagtt ggccagcatc ttcctggacc agaagtgcca
      901 aaatatcctg ca
//
