LOCUS       sequence-52803-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ALDH18A1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatggt aaagcctgca ggccctactg ttgagcagca
      241 gggagaaatg gcgcgatctg gaggaaggat gttggccacc ttggaacctg agcagagagc
      301 agaaattatc catcatctgg ctgatctgtt gacggaccag cgtgatgaga tcctgttagc
      361 caacaaaaaa gacttggagg aggcagaggg gagacttgca gctcctctgc tgaaacgttt
      421 aagcctctcc acatccaaat tgaacagcct ggccatcggt ctgcgacaga tcgcagcctc
      481 ctcccaggac agcgtgggac gtgttttgcg ccgcacccga atcgccaaaa acttggaact
      541 ggaacaagtg actgtcccaa ttggagttct gctggtgatc tttgaatctc gtcctgactg
      601 tctaccccag gtggcagctt tggctatcgc aagtggcaat ggcttgttac tcaaaggagg
      661 gaaggaggct gcacacagca accggattct ccacctcctg acccaggagg ctctctcaat
      721 ccatggagtc aaggaggccg tgcaactggt gaataccaga gaagaagttg aagatctttg
      781 ccgcctagac aaaatgatag atctgatcat tccacgtggc tcttcccagc tggtcagaga
      841 catccagaaa gc
//
