LOCUS       sequence-52804-e         556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 556)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SLC9A3R2
FEATURES             Location/Qualifiers
     source          1..556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..86
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        67..85
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             158..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgtggcgcgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
       61 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
      121 taattttgtt taactttaag aaggagatat acatatgcac catcatcatc atcattcttc
      181 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgccgcgcc tgtgccgctt
      241 ggtgcgcgga gagcagggct acggcttcca cctgcacggc gagaagggcc gccgcgggca
      301 gttcatccgg cgcgtggaac ccggttcccc cgccgaggcc gccgcgctgc gcgctgggga
      361 ccgcctggtc gaggtcaacg gcgtcaacgt ggagggcgag acgcaccacc aggtggtgca
      421 aaggatcaag gctgtggagg ggcagactcg gctgctggtg gtggaccagg aggacacttc
      481 tgtttgacag taaaggtgga tacggatccg aattcgagct ccgtcgacag ttgcggcgcc
      541 tcgacaccac caccac
//
