LOCUS       sequence-52807-e       15060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15060)
  AUTHORS   Shibue T, Weinberg RA
  TITLE     Integrin beta1-focal adhesion kinase signaling directs the 
            proliferation of metastatic cancer cells disseminated in the lungs.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10290-5. Epub 2009 Jun
            5.
  PUBMED    19502425
REFERENCE   2  (bases 1 to 15060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-06-23"; volume: "106(25)"; pages: 
            "10290-5. Epub 2009 Jun 5"
COMMENT     Sequence Label: pLV-neo-CD2-FAK
FEATURES             Location/Qualifiers
     source          1..15060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7462..8028
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7479..8030
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7623..7640)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7850..7869
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8081..8875
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDRLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8135..8154)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8745..8764
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8890..9478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8943..8963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9361..9372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9622..9655)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9675..9855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9880..9898)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9881..9898)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10188..10207)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10421..10440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10540..10562
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10600..10618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10686..10790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10791..10859,10860..11651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11009..11028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11822..12410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12311..12330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12564..12581
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12651..12671)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12656..12985
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12836..12971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          14119..14184
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14314..14334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14759..14893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14796..14815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14850..14869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaactacggg atccacgcgt gccaccatga gctttccctg taaatttgta
     3541 gccagcttcc ttctgatttt caatgtttct tccaaaggtg cagtctccaa agagattacg
     3601 aatgccttgg aaacctgggg tgccttgggt caggacatca acttggacat tcctagtttt
     3661 caaatgagtg atgatattga cgatataaaa tgggaaaaaa cttcagacaa gaaaaagatt
     3721 gcacaattca gaaaagagaa agagactttc aaggaaaaag atacatataa gctatttaaa
     3781 aatggaactc tgaaaattaa gcatctgaag accgatgatc aggatatcta caaggtatca
     3841 atatatgata caaaaggaaa aaatgtgttg gaaaaaatat ttgatttgaa gattcaagag
     3901 agggtctcaa aaccaaagat ctcctggact tgtatcaaca caaccctgac ctgtgaggta
     3961 atgaatggaa ctgaccccga attaaacctg tatcaagatg ggaaacatct aaaactttct
     4021 cagagggtca tcacacacaa gtggaccacc agcctgagtg caaaattcaa gtgcacagca
     4081 gggaacaaag tcagcaagga atccagtgtc gagcctgtca gctgtccaga gaaaggtctg
     4141 gacatctatc tcatcattgg catatgtgga ggaggcagcc tcttgatggt ctttgtggca
     4201 ctgctcgttt tctatatcac caaaaggaaa aaacagagga gtcggagaaa atcgatggca
     4261 gctgcttacc ttgaccccaa cttgaatcac acaccaaatt cgagtactaa gactcacctg
     4321 ggtactggta tggaacgttc tcctggtgca atggagcgag tattaaaggt ctttcattat
     4381 tttgaaagca atagtgagcc aaccacctgg gccagtatta tcaggcatgg agatgctact
     4441 gatgtcaggg gcatcattca gaagatagtg gacagtcaca aagtaaagca tgtggcctgc
     4501 tatggattcc gcctcagtca cctgcggtca gaggaggttc actggcttca cgtggatatg
     4561 ggcgtctcca gtgtgaggga gaagtatgag cttgctcacc caccagagga gtggaaatat
     4621 gaattgagaa ttcgttattt gccaaaagga tttctaaacc agtttactga agataagcca
     4681 actttgaatt tcttctatca acaggtgaag agcgattata tgttagagat agctgatcaa
     4741 gtggaccagg aaattgcttt gaagttgggt tgtctagaaa tacggcgatc atactgggag
     4801 atgcggggca atgcactaga aaagaagtct aactatgaag tattagaaaa agatgttggt
     4861 ttaaagcgat tttttcctaa gagtttactg gattctgtca aggccaaaac actaagaaaa
     4921 ctgatccaac aaacatttag acaatttgcc aaccttaata gagaagaaag tattctgaaa
     4981 ttctttgaga tcctgtctcc agtctacaga tttgataagg aatgcttcaa gtgtgctctt
     5041 ggttcaagct ggattatttc agtggaactg gcaatcggcc cagaagaagg aatcagttac
     5101 ctaacggaca agggctgcaa tcccacacat cttgctgact tcactcaagt gcaaaccatt
     5161 cagtattcaa acagtgaaga caaggacaga aaaggaatgc tacaactaaa aatagcaggt
     5221 gcacccgagc ctctgacagt gacggcacca tccctaacca ttgcggagaa tatggctgac
     5281 ctaatagatg ggtactgccg gctggtgaat ggaacctcgc agtcatttat catcagacct
     5341 cagaaagaag gtgaacgggc tttgccatca ataccaaagt tggccaacag cgaaaagcaa
     5401 ggcatgcgga cacacgccgt ctctgtgtca gaaacagatg attatgctga gattatagat
     5461 gaagaagata cttacaccat gccctcaacc agggattatg agattcaaag agaaagaata
     5521 gaacttggac gatgtattgg agaaggccaa tttggagatg tacatcaagg catttatatg
     5581 agtccagaga atccagcttt ggcggttgca attaaaacat gtaaaaactg tacttcggac
     5641 agcgtgagag agaaatttct tcaagaagcc ttaacaatgc gtcagtttga ccatcctcat
     5701 attgtgaagc tgattggagt catcacagag aatcctgtct ggataatcat ggagctgtgc
     5761 acacttggag agctgaggtc atttttgcaa gtaaggaaat acagtttgga tctagcatct
     5821 ttgatcctgt atgcctatca gcttagtaca gctcttgcat atctagagag caaaagattt
     5881 gtacacaggg acattgctgc tcggaatgtt ctggtgtcct caaatgattg tgtaaaatta
     5941 ggagactttg gattatcccg atatatggaa gatagtactt actacaaagc ttccaaagga
     6001 aaattgccta ttaaatggat ggctccagag tcaatcaatt ttcgacgttt tacctcagct
     6061 agtgacgtat ggatgtttgg tgtgtgtatg tgggagatac tgatgcatgg tgtgaagcct
     6121 tttcaaggag tgaagaacaa tgatgtaatc ggtcgaattg aaaatgggga aagattacca
     6181 atgcctccaa attgtcctcc taccctctac agccttatga cgaaatgctg ggcctatgac
     6241 cccagcaggc ggcccaggtt tactgaactt aaagctcagc tcagcacaat cctggaggaa
     6301 gagaaggctc agcaagaaga gcgcatgagg atggagtcca gaagacaggc cacagtgtcc
     6361 tgggactccg gagggtctga tgaagcaccg cccaagccca gcagaccggg ttatcccagt
     6421 ccgaggtcca gcgaaggatt ttatcccagc ccacagcaca tggtacaaac caatcattac
     6481 caggtttctg gctaccctgg ttcacatgga atcacagcca tggctggcag catctatcca
     6541 ggtcaggcat ctcttttgga ccaaacagat tcatggaatc atagacctca ggagatagca
     6601 atgtggcagc ccaatgtgga ggactctaca gtattggacc tgcgagggat tgggcaagtg
     6661 ttgccaaccc atctgatgga agagcgtcta atccgacagc aacaggaaat ggaagaagat
     6721 cagcgctggc tggaaaaaga ggaaagattt ctgaaacctg atgtgagact ctctcgaggc
     6781 agtattgaca gggaggatgg aagtcttcag ggtccgattg gaaaccaaca tatatatcag
     6841 cctgtgggta aaccagatcc tgcagctcca ccaaagaaac cgcctcgccc tggagctccc
     6901 ggtcatctgg gaagccttgc cagcctcagc agccctgctg acagctacaa cgagggtgtc
     6961 aagcttcagc cccaggaaat cagcccccct cctactgcca acctggaccg gtcgaatgat
     7021 aaggtgtacg agaatgtgac gggcctggtg aaagctgtca tcgagatgtc cagtaaaatc
     7081 cagccagccc caccagagga gtatgtccct atggtgaagg aagtcggctt ggccctgagg
     7141 acattattgg ccactgtgga tgagaccatt cccctcctac cagccagcac ccaccgagag
     7201 attgagatgg cacagaagct attgaactct gacctgggtg agctcatcaa caagatgaaa
     7261 ctggcccagc agtatgtcat gaccagcctc cagcaagagt acaaaaagca aatgctgact
     7321 gctgctcacg ccctggctgt ggatgccaaa aacttactcg atgtcattga ccaagcaaga
     7381 ctgaaaatgc ttgggcagac gagaccacac tgacggccgg gtgttgtaat tcattaagca
     7441 ttctgccgac accgggtaaa ttccgccccc cccccctaac gttactggcc gaagccgctt
     7501 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     7561 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     7621 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     7681 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     7741 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     7801 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     7861 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     7921 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
     7981 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata ccatggccga
     8041 attcttcaaa tatgtatccg ctcatgagac aataaccctg atgattgaac aagatagatt
     8101 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     8161 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     8221 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     8281 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     8341 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     8401 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     8461 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     8521 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     8581 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     8641 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     8701 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8761 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8821 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaactag
     8881 tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8941 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     9001 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9061 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9121 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9181 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9241 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     9301 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9361 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9421 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     9481 cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9541 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     9601 tgggatcaat tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc
     9661 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9721 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9781 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9841 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     9901 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     9961 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
    10021 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
    10081 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
    10141 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
    10201 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
    10261 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
    10321 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
    10381 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
    10441 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    10501 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    10561 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
    10621 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
    10681 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10741 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    10801 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10861 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10921 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10981 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    11041 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11101 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11161 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11221 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11281 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11341 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11401 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11461 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11521 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11581 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11641 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11701 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    11761 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    11821 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    11881 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11941 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
    12001 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    12061 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    12121 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    12181 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
    12241 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    12301 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    12361 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    12421 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    12481 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    12541 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    12601 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
    12661 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    12721 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    12781 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    12841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    12901 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    12961 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
    13021 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
    13081 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
    13141 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
    13201 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    13261 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
    13321 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    13381 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    13441 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    13501 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    13561 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    13621 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    13681 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    13741 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    13801 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    13861 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    13921 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    13981 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    14041 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
    14101 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
    14161 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
    14221 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
    14281 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
    14341 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    14401 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    14461 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    14521 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    14581 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    14641 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    14701 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    14761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    14821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    14881 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    14941 ataccctatt accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct
    15001 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
//
