LOCUS       sequence-52812-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG634
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             303..323
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             336..380
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(382..406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtttaactt ggacttacta actaacggat tatatttaaa ttttcagaac attaacatgg
       61 cactactact agaaggtgga ggtcactacc tttgcgattt taaaactact tacaaagcaa
      121 agaaggtcgt ccaacttcca gatgcacact ttgttgatca cagaattgaa atactaggaa
      181 acgattcgga ttacaacaaa gttaagctat acgaacacgc agttgcaaga tacagtcctc
      241 taccaagtca agcatggtgc ccgggggatc ggtggagctc caccggtggc ggccgctcga
      301 gagagaatct ttattttcag ggcgccgcca aattcaaaga aaccgctgct gctaaattcg
      361 aacgccagca catggacagc cacccagctt tcttgtacaa agtggagcat ttccatgaac
      421 atcgtggcga gaagttcggt cgtcgtggat tcccaattcc agaaactgac agtcaacaac
      481 cacccaacta caagactcgt ctttgcatga tgcacgcatc tggaatcaaa ccatgtgata
      541 tgggtgctcg atgcaagttt gctcatgggc tcaaagagct cagagctact gatgctccgg
      601 ctcgctatcc gaacaacaag tacaagacaa agctgtgcaa gaactttgcg cgtggaggaa
      661 ctggattctg cccgtatgga cttcgttgcg agtttgttca tccaacggac aaggaattcc
      721 agaatattcc tccatatgtg agtttccact tttgctcatc atttttgaaa ctttaacata
      781 ttttcagcag cgcatgtctc acgatgacca ggattacgac cagga
//
