LOCUS       sequence-52835-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Watt S, Mata J, Lopez-Maury L, Marguerat S, Burns G, Bahler J
  TITLE     urg1: a uracil-regulatable promoter system for fission yeast with 
            short induction and repression times.
  JOURNAL   PLoS One. 2008 Jan 16;3(1):e1428.
  PUBMED    18197241
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-01-16"; volume: "3(1)"; pages: "e1428"
COMMENT     Sequence Label: pFA6a-kanMX6-Purg1-3HA
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(55..242)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     163..187
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(275..364)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tcgtacgctg caggtcgacg gatctatatt accctgttat ccctagcgga tctgccggta
       61 gaggtgtggt caataagagc gacctcatac tatacctgag aaagcaacct gacctacagg
      121 aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg
      181 tatacactta ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc
      241 gcttatttag aagtggcgcg cctcagcact gagcagcgta atctggaacg tcatatggat
      301 aggatcctgc atagtccggg acgtcatagg gatagcccgc atagtcagga acatcgtatg
      361 ggtaaaagat gttaattaac ctttgttcag tggcaagcat attgaattag ttctaattta
      421 gtaattgaag aaaacggata agtaaaacaa caagaaaaat gacaaacgaa tgttcaatgg
      481 gtagaaagaa attcgtttca caaaattagc ctttaaacaa agtgaattga gtaaggcagc
      541 taaagtaatg aatgaaacct ttgcaattga tgttgaaccg tatggatttc ctcccttatt
      601 tatagccttc cagatactat caatgaaagc gctagctcgc atgataccag ataccacgcg
      661 gacttgccgc gggcacttcc tcgtcagcga accgaaaaca ataccttgtt tacatcgttg
      721 cgccttctta gaccttacga agtcgcgggg aagggtcgtg cacgcatgtt ctcgagcggc
      781 gctaccgcgg gcaatagccg acgcaagtca ttgtttagaa gaaaaaaaat ttttttgtaa
      841 tgtaaacaat tggaattggt tgatgtcagt aagcaaatat tcccatttac aggaaaagaa
      901 tagaacatca ttcttaca
//
