LOCUS       sequence-52837-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HMGCS2
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             45..371
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             378..398
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aaggagatat acatatgcac catcatcatc atcattcttc tggtatgagc gataaaatta
       61 ttcacctgac tgacgacagt tttgacacgg atgtactcaa agcggacggg gcgatcctcg
      121 tcgatttctg ggcagagtgg tgcggtccgt gcaaaatgat cgccccgatt ctggatgaaa
      181 tcgctgacga atatcagggc aaactgaccg ttgcaaaact gaacatcgat caaaaccctg
      241 gcactgcgcc gaaatatggc atccgtggta tcccgactct gctgctgttc aaaaacggtg
      301 aagtggcggc aaccaaagtg ggcgcactgt ctaaaggtca gttgaaagag ttcctcgacg
      361 ctaacctggc cggtaccgag aacttgtact tccaatccat gccaaaggac gtgggcatcc
      421 tggccctgga ggtctacttc ccagcccaat atgtggacca aactgacctg gagaagtata
      481 acaatgtgga agcaggaaag tatacagtgg gcttgggcca gacccgtatg ggcttctgct
      541 cagtccaaga ggacatcaac tccctgtgcc tgacggtggt gcaacggctg atggagcgca
      601 tacagctccc atgggactct gtgggcaggc tggaagtagg cactgagacc atcattgaca
      661 agtccaaagc tgtcaaaaca gtgctcatgg aactcttcca ggattcaggc aatactgata
      721 ttgagggcat agataccacc aatgcctgct acggtggtac tgcctccctc ttcaatgctg
      781 ccaactggat ggagtccagt tcctgggatg gtcgttatgc catggtggtc tgtggagaca
      841 ttgccgtcta tcccagtggt aatgctcgtc ccacaggtgg ggccggagct gtggctatgc
      901 tgattgggcc caaggccc
//
