LOCUS       sequence-52839-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPSB2
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             729..746
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(798..816)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct gggtaccgag
       61 aacctgtact tccaatccat gcccgagggc ttggaagagc tgctgtctgc accccctcct
      121 gacctggggg cccagcggcg ccacggttgg aaccccaaag actgttcaga gaacatcgag
      181 gtcaaggaag gagggttgta ctttgagcgg cggcccgtgg cccagagcac tgatggggcc
      241 cggggtaaga ggggctattc aaggggcctg cacgcctggg agatcagctg gcccctagag
      301 cagaggggca cgcatgccgt ggtgggcgtg gccacggccc tcgccccgct gcagactgac
      361 cactacgcgg cgctgctggg cagcaacagc gagtcgtggg gctgggacat cgggcggggg
      421 aagctgtacc atcagagcaa ggggcccgga gccccccagt atccagcggg aactcagggt
      481 gagcagctgg aggtgccaga gagactgctg gtggttctgg acatggagga gggaactctg
      541 ggctacgcta ttgggggcac ctacctgggg ccagcattcc gcggactgaa gggcaggacc
      601 ctctatccgg cagtaagcgc tgtctggggc cagtgccagg tccgcatccg ctacctgggc
      661 gaatgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
      721 cactcgagca ccaccaccac caccactgag atccggctgc taacaagccc gaaaggaagc
      781 tgagttggct gctgccaccg ctgagcaata actagcataa acccttgggg cctctaacgg
      841 gtcttgaggg ttttttgctg aa
//
