LOCUS       sequence-52842-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TATDN3
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatggg cgtaggcttg gtggactgtc actgccacct
      241 ctccgccccg gactttgacc gcgatttgga tgatgtgttg gagaaagcca agaaggccaa
      301 tgttgtggcc cttgtggcag ttgccgaaca ttcaggagaa tttgaaaaga ttatgcaact
      361 ttcagaaagg tataatgggt ttgtcctgcc atgcttgggt gttcatccag ttcaaggact
      421 tccaccagaa gaccaaagaa gtgtcacact aaaggatttg gatgtagctt tgcccattat
      481 tgagaattat aaggatcggt tgttggcaat tggagaggtt ggactagatt tctcccccag
      541 atttgctggc actggtgaac agaaggaaga gcaaagacaa gtcctaatca gacagatcca
      601 gttagccaaa agactaaatt tgcctgtaaa tgtgcactca cgctctgctg gaagacctac
      661 catcaacctt ttacaagagc aaggtgctga gaaggtactg ctgcatgcat ttgatggtcg
      721 gccatctgta gccatggaag gagtaagagc tgggtacttc ttctcaattc ccccttctat
      781 cataagaagt ggacagaagc agaaacttgt gaaacaattg cctttaactt ctatatgctt
      841 agaaacagat tcacctgcac taggaccaga aaaacaggta cggaatgagc cctgg
//
