LOCUS       sequence-52843-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LACTB2
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             184..204
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggancg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt agatctgggt
      181 accgagaacc tgtacttcca atccatggct gctgtactgc agcgcgtcga gcggctgtcc
      241 aatcgagtcg tgcgtgtgtt gggctgtaac ccgggtccca tgaccctcca aggcaccaac
      301 acctacctag tggggaccgg ccccaggaga atcctcattg acactggaga accagcaatt
      361 ccagaataca tcagctgttt aaagcaggct ctaactgaat ttaacacagc aatccaggaa
      421 attgtagtga ctcactggca ccgagatcat tctggaggca taggagatat ttgtaaaagc
      481 atcaataatg acactaccta ttgcattaaa aaactcccac ggaatcctca gagagaagaa
      541 attataggaa atggagagca acaatatgtt tatctgaaag atggagatgt gattaagact
      601 gagggagcca ctctaagagt tctatatacc cctggccaca ctgatgatca catggctcta
      661 ctcttagaag aggaaaatgc tatcttttct ggagattgca tcctagggga aggaacaacg
      721 gtatttgaag acctctatga ttatatgaac tctttaaaag agttattgaa aatcaaagct
      781 gatattatat atccaggaca tggcccagta attcataatg ctgaagctaa aattcacata
      841 ca
//
