LOCUS       sequence-52862-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     Kaplan lab pGL3-IL-4 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL3-IL-4
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    454..545
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(486..505)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    559..607
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg gaatgccaag
       61 cttagagaca attaagttta tcaagagatg ctaacaatgc aatgctggca gaggtctctc
      121 tatatataga gttaaagttg ctgaaaccaa gggaaaatga gtttacattg gaaaatttta
      181 ttacaccaga ttgtcagtca ttctgggcca atcagcacct ctcttccagg agaaaaaaga
      241 gaaacaggta aattttcctg tgaaatcaga ccaattggaa aatgaaacac ccctcccccc
      301 cctttttttt taaatctaca aagtttcagc ataggaaatt acaccataat cggcctttca
      361 gactaatctt atcagcgtag ggttgccact ggctctcctc ctgcagagac tcgagcccgg
      421 gctagcacgc gtaagagctc ggtacctatc gatagagaaa tgttctggca cctgcacttg
      481 cactggggac agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt
      541 atgttagtac tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata
      601 ttttattgcg gccgctccaa gtacctcccg taccttaata ttacttactt atcatggtag
      661 cttgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      721 cctgaatggc gaatggcaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      781 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaa
//
