LOCUS       sequence-52868-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACBD7
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             428..445
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(498..516)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      512..559
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(683..702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     893..914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat ggccctgcag gctgattttg
      121 acagggctgc agaagatgtg aggaagctga aagcaagacc agatgatgga gaactgaaag
      181 aactctatgg gctttacaaa caagcaatag ttggagacat taatattgcg tgtccaggaa
      241 tgctagattt gaaaggcaaa gccaaatggg aagcatggaa cctcaaaaaa gggttgtcga
      301 cggaagatgc gacgagtgcc tatatttcta aagcaaagga gctgatagaa aaatacggaa
      361 tttgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
      421 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      481 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      541 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      781 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      901 ttgttccaaa ctggaacaac actcaaccct atct
//
