LOCUS       sequence-52872-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CRYL1
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg gccggctgcg tggtgatcgt
      121 tggcagtgga gtcattgggc gaagctgggc catgctgttt gccagtggag gcttccaggt
      181 gaaactctat gacattgagc aacagcagat aaggaacgcc ctggaaaaca tcagaaagga
      241 gatgaagttg ctggagcagg caggttctct gaaaggctcc ctgagtgtgg aagagcagct
      301 gtcactcatc agtggttgtc ccaatatcca agaagcagta gagggtgcca tgcacattca
      361 ggaatgtgtt ccagaagatc tagaactgaa gaagaagatt tttgctcagt tagattccat
      421 cattgatgat cgagtgatct taagcagttc cacttcttgt ctcatgcctt ccaagttgtt
      481 tgctggcttg gtccatgtga agcaatgcat cgtggctcat cctgtgaatc cgccatacta
      541 catcccgctg gttgagctgg tcccccaccc ggagacggcc cctacgacag tggacagaac
      601 ccacgccctg atgaagaaga ttggacagtg ccccatgcga gtccagaagg aggtggccgg
      661 cttcgttctg aaccgcctgc aatatgcaat catcagcgag gcctggcggc tagtggagga
      721 aggaatcgtg tctcctagtg acctggacct tgtcatgtca gaagggttgg gcatgcggta
      781 tgcattcatt ggacccctgg aaaccatgca tctcaatgca gaaggtatgt taagctactg
      841 cgacagatac agcgaaggca taaaacatgt cctacagact tttggaccca ttccagagtt
      901 ttccagg
//
