LOCUS       sequence-52877-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CA8
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat ggcggacctg agcttcatcg
      121 aagataccgt cgccttcccc gagaaggaag aggatgagga ggaagaagag gagggtgtgg
      181 agtggggcta cgaggaaggt gttgagtggg gtctggtgtt tcctgatgct aatggggaat
      241 accagtctcc tattaaccta aactcaagag aggctaggta tgacccctcg ctgttggatg
      301 tccgcctctc cccaaattat gtggtgtgcc gagactgtga agtcaccaat gatggacata
      361 ccattcaggt tatcctgaag tcaaaatcag ttctttcggg aggaccattg cctcaagggc
      421 atgaatttga actgtacgaa gtgagatttc actggggaag agaaaaccag cgtggttctg
      481 agcacacggt taatttcaaa gcttttccca tggagctcca tctgatccac tggaactcca
      541 ctctgtttgg cagcattgat gaggctgtgg ggaagccgca cggaatcgcc atcattgctc
      601 tgtttgttca gataggaaag gaacatgttg gcttgaaggc tgtgactgaa atcctccaag
      661 atattcagta taaggggaag tccaaaacaa taccttgctt taatcctaac actttattac
      721 cagaccctct gctgcgggat tactgggtgt atgaaggctc tctcaccatc ccaccttgca
      781 gtgaaggtgt cacctggata ttattccgat accctt
//
