LOCUS       sequence-52888-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SCRIB
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             454..471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(524..542)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      538..585
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(709..728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggaagaac tgaccctgac
      121 catcctgcgt cagaccggcg gcctgggcat ttcgatcgcc ggcggcaaag gtagtacccc
      181 gtataaaggc gatgatgaag gcatttttat ctcccgtgtt agcgaagaag gtccggccgc
      241 tcgtgctggt gtccgcgttg gggataaact gctggaagtt aacggtgtgg ctctgcaggg
      301 cgcggagcat catgaggcgg tggaagcgct gcgtggggcg ggtaccgccg tgcaaatgcg
      361 tgtctggcgc gaacgtgaaa ccagcgtttg acagtaaagg tggatacgga tccgaattcg
      421 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      481 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      541 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      601 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      661 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      721 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      781 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      841 gtgatggttc acgtagtggg ccatcgccct gatagacgg
//
