LOCUS       sequence-52902-e         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for N-term FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR4-FLAG (w210-2)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(201..300)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(412..431)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(490..509)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggctccac catggattac aaggacgatg
      121 acgacaaact cggatccatg ggaaccaatt cagtcgactg gatccggtac cgaattcgcg
      181 gccgcactcg agatatctag acccagcttt cttgtacaaa gttggcatta taagaaagca
      241 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      301 ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
      361 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
      421 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
      481 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
      541 gcttgtatgg gaagcccgat gcgccagagt
//
