LOCUS       sequence-52903-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACBD5
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             462..479
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(532..550)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      546..593
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      630..1010
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(717..736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     927..948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgtccgtgca cgagactagg
      121 tttgaggcgg ccgtgaaggt gatccagagt ttgccgaaga atggttcatt ccagccaaca
      181 aatgaaatga tgcttaaatt ttatagcttc tataagcagg caactgaagg accctgtaaa
      241 ctttcaaggc ctggattttg ggatcctatt ggaagatata aatgggatgc ttggagttca
      301 ctgggtgata tgaccaaaga ggaagccatg attgcatatg ttgaagaaat gaaaaagatt
      361 attgaaacta tgccaatgac tgagaaagtt gaagaatgac agtaaaggtg gatacggatc
      421 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      481 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      541 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      601 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      841 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      901 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      961 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     1021 aa
//
