LOCUS       sequence-52909-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             525..542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(595..613)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      609..656
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(780..799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc tggtgtagat
       61 ctgggtaccg agaacctgta cttccaatcc atgagcggca ttagcccgaa aaaaagcaaa
      121 tatatgaccc cgatgcagca gaaactgaac gaagtgtatg aagcggtgaa aaactatacc
      181 gataaacgcg gtcgccgtct gagcgccatt tttctgcgcc tgccgagccg cagcgaactg
      241 ccggattatt atctgaccat taaaaaaccg atggatatgg aaaaaattcg cagccacatg
      301 atggcgaata aatatcagga tattgatagc atggttgaag attttgttat gatgttcaat
      361 aacgcgtgca cctataacga accggaaagc ctgatctata aagatgcgct ggtgctgcat
      421 aaagtgctgc tggaaacccg ccgcgatctg gaaggcgatt gacagtaaag gtggatacgg
      481 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      541 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      601 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      661 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
//
