LOCUS       sequence-52910-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BAZ2B
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             508..525
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(578..596)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      592..639
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttgtttactt taagaaggag atatacatat gcaccatcat catcaccatt cttctggtgt
       61 agatctgggt accgagaacc tgtacttcca atccatgagc gtgaaaaaac cgaaacgcga
      121 tgatagcaaa gatctggcgc tgtgcagcat gattctgacc gaaatggaaa cccatgaaga
      181 tgcgtggccg tttctgctgc cggtgaacct gaaactggtt ccgggctata aaaaagtgat
      241 taaaaaaccg atggatttta gcaccattcg tgaaaaactg agcagcggtc agtatccgaa
      301 cctggaaacc tttgcgctgg atgtgcgtct ggtgtttgat aactgcgaaa cctttaacga
      361 agatgatagc gatattggcc gtgccggtca taacatgcgc aaatattttg aaaaaaaatg
      421 gaccgatacc tttaaagtta gctgacagta aaggtggata cggatccgaa ttcgagctcc
      481 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      541 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      601 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      661 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      721 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      841 ttagggttcc gatttagtgc tttacggcac ctcgaacccc caaaaaactt gattaagggg
      901 tgatggttca cgttagtggg gccatcg
//
