LOCUS       sequence-52915-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: KAT2B
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             501..518
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(571..589)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      585..632
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(756..775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga tctgggtacc
       61 gagaacctgt acttccaatc catgggaaaa gagaaaagta aagagcccag agaccctgac
      121 cagctttaca gcacgctcaa gagcatcctc cagcaggtga agagccatca aagcgcttgg
      181 cccttcatgg aacctgtgaa gagaacagaa gctccaggat attatgaagt tataaggttc
      241 cccatggatc tgaaaaccat gagtgaacgc ctcaagaata ggtactacgt gtctaagaaa
      301 ttattcatgg cagacttaca gcgagtcttt accaattgca aagagtacaa cccccctgag
      361 agtgaatact acaaatgtgc caatatcctg gagaaattct tcttcagtaa aattaaggaa
      421 gctggattaa ttgactgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      481 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      541 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      601 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      661 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggg
//
