LOCUS       sequence-52921-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACAD11
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..388
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             395..415
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aattttgttt actttagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tatgagcgat aaaattattc acctgactga cgacagtttt gacacggatg tactcaaagc
      121 ggacggggcg atcctcgtcg atttctgggc agagtggtgc ggtccgtgca aaatgatcgc
      181 cccgattctg gatgaaatcg ctgacgaata tcagggcaaa ctgaccgttg caaaactgaa
      241 catcgatcaa aaccctggca ctgcgccgaa atatggcatc cgtggtatcc cgactctgct
      301 gctgttcaaa aacggtgaag tggcggcaac caaagtgggc gcactgtcta aaggtcagtt
      361 gaaagagttc ctcgacgcta acctggccgg taccgagaac ttgtacttcc aatccatgtc
      421 caaacgaact ttcagtactg tactaccaca gattgatact actggacagt tgtttgtaca
      481 gactcggaaa ggtcaggaag ttcttattaa ggtgaagcat ttcatgaaac aacacattct
      541 tccagctgaa aaggaggtaa ctgagttcta tgttcaaaat gaaaattcag tggacaagtg
      601 gggaaaacct ttagtgattg ataaactcaa ggaaatggcc aaagtcgagg gtctctggaa
      661 cttgtttttg ccagctgtca gcggactcag ccacgtggac tatgccttga ttgctgaaga
      721 aacaggaaaa tgcttttttg ctccagatgt ctttaactgc caagcaccag acacagggaa
      781 tatggaggtt ctgcacctgt atggaagtga ggaacagaag aaacagtggc ttgagcctct
      841 tcttcaaggg aacattacct cttgcttctg tatgacagaa cctgatgtag cttcaagtga
      901 tgccacgaat attgaatgca gcatccaacg agatgaagat agctatgtaa ttaacggcaa
      961 aaaatgtg
//
