LOCUS       sequence-52934-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K,
            Hirabayashi S, Bao Y, Hidaka S, Hirata Y, Hata Y
  TITLE     Hippo pathway-dependent and -independent roles of RASSF6.
  JOURNAL   Sci Signal. 2009 Sep 29;2(90):ra59.
  PUBMED    19797269
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2009-09-29"; volume: "2(90)"; pages: "ra59"
COMMENT     Sequence Label: pCIneoFH human RASSF6
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          166..298
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     281..300
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        343..361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             397..420
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             427..444
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagctcgttt agtgaccgtc agatcactag aagctttatt gcggtagttt atcacagtta
       61 aattgctaaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagtgact
      121 ctcttaaggt agccttgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
      181 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
      241 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
      301 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
      361 gctagcagat ctcgagatct gctagccggg gccatggact acaaagacga tgacgacaag
      421 gaattgcacc atcatcatca tcatgaattg actatgatgg ctcaccagta cccctcttgg
      481 atcttcatta atgagaagac attcataacc agggaacaac ttaattcttt attgaagacc
      541 tataacattt tttatgagaa ccagaaaaat ctgcatattt tatatggaga gactgaagat
      601 ggcaaactaa ttgttgaagg aatgctggac attttctggg gagtaaaacg acctatacag
      661 ctaaaaatac aagatgagaa gccattctct tcttttacta gtatgaagtc atcagacgtc
      721 ttctccagca aaggaatgac acgctggggg gaatttgacg atctctatcg tattagtgag
      781 ctggacagga cccagattcc tatgtctgaa aaaaggaatt cccaggaaga ctatttatct
      841 tatcacagc
//
