LOCUS       sequence-52950-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for cloning your gene fused to mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  TITLE     Fluorescent Protein Plasmids
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-mRFP
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..699)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(22..699)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(84..107)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(282..301)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     551..569
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tatagaatag ggccctctag attaggcgcc ggtggagtgg cggccctcgg cgcgctcgta
       61 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttga tgtcggtctt
      121 gtaggcgccg ggcagctgca cgggcttctt ggccatgtag gtggtcttga cctcggcgtc
      181 gtagtggccg ccgtccttca gcttcagcct catcttgatc tcgcccttca gggcgccgtc
      241 ctcggggtac atccgctcgg tggaggcctc ccagcccatg gtcttcttct gcattacggg
      301 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
      361 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
      421 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
      481 cacgtaggcc ttggagccgt actggaactg aggggacagg atgtcccagg cgaagggcag
      541 ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
      601 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa
      661 gcgcatgaac tccttgatga cgtcctcgga ggaggccatc
//
