LOCUS       sequence-52952-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00606
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(327..426)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(443..462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(444..462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(467..483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(467..483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(585..604)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(663..682)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg caccatggca cgtggacacc agaagatcca
      121 gtcgcaggcg aaggcctccg agaagcaggc caaactaaag aagcaacaag gacacagtgc
      181 caacgaccag aagaaggcgg cccaaaaggc acttgtctat gtgtgcgccg tttgcaagtc
      241 gcaaatgccc gatcccaaga cgtataaaca gcacttcgag aacaagcatc ccaagaacga
      301 catacccgag gagctgaagg aggtcttgcc aactttcttg tacaaagttg gcattataag
      361 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      421 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
      481 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      541 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
      601 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
      661 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      721 tgggaagccc gatgcgccag agttgtttct gaa
//
