LOCUS       sequence-52975-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus/LRRK2 1867-2186
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..93
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatata
       61 ggatccatgc accaccatca ccaccatcac cacgagaacc tttatttcca aggaaacatc
      121 atgctcaaca acgatgagct cgaatttgaa caagctccag aattcctgct cggcgacggc
      181 tctttcggct ccgtataccg tgctgcttat gaaggcgaag aagtcgccgt aaagatcttc
      241 aacaagcaca cctcgctccg tctgctccgt caggaactgg tcgttctgtg tcatctgcac
      301 catccgagcc tcatctccct gctggccgct ggcatccgtc ctcgtatgct ggtgatggag
      361 ctggcatcca agggctctct cgaccgcctg ctgcagcagg acaaggcttc tctgacccgt
      421 actctgcagc atcgtatcgc tctgcatgta gctgacggcc tgcgttatct tcattctgca
      481 atgatcattt atcgcgacct gaaacctcat aatgtactgc tgtttactct gtacccgaac
      541 gctgccatca tcgctaagat tgcagactat ggtattgcgc agtactgctg ccgcatgggc
      601 attaaaacaa gcgaaggtac gccgggtttc cgtgctccag aagtggctcg tggcaacgtt
      661 atttacaacc agcaagctga cgtttactcg ttcggtctgc tcctgtacga tatcctgact
      721 accggcggcc gtatcgtaga aggtctgaag ttccctaacg aattcgacga actggaaatc
      781 cagggcaagc tgccagaccc ggttaaagaa tacggttgcg ctccgtggcc gatggt
//
