LOCUS       sequence-52982-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX5
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             516..533
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(586..604)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      600..647
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(771..790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgggcgcggg cggcggcggc
      121 tcggcggagc agttggacgc gctggtgaag aaggacaagg tggtggtctt cctcaagggg
      181 acgccggagc agccccagtg cggcttcagc aacgccgtgg tgcagatcct gcggctgcac
      241 ggcgtccgcg attacgcggc ctacaacgtg ctggacgacc cggagctccg acaaggcatt
      301 aaagactatt ccaactggcc caccatcccg caagtgtacc tcaatggcga gtttgtaggg
      361 ggctgtgaca ttcttctgca gatgcaccag aatggggact tggtggaaga actgaaaaag
      421 ctggggatcc actccgccct tttagatgaa tgacagtaaa ggtggatacg gatccgaatt
      481 cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      541 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      601 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      661 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      841 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
//
