LOCUS       sequence-52984-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TXNRD3
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             359..379
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             380..397
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             398..421
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             471..488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(541..559)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      555..602
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(726..745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaatattttg tttaccttaa gaaggagata tactatggcg cgcgaagaac tgcgtcgtca
       61 tctggttgga ctgattgagc gctcccgtgt ggtaatcttt tcaaagtctt attgtccgca
      121 tagtacgcgt gttaaagaac tgttttccag cctgggtgta gaatgcaatg tgctggagct
      181 ggaccaggtg gatgatggtg cccgcgttca ggaagtcctg tctgaaatta ccaaccagaa
      241 aacggtcccg aacattttcg tgaataaagt gcacgtcggt ggctgcgatc agacttttca
      301 ggcatatcag agtgggctgc tgcagaaact gctgcaagaa gacctggcct atgatgcaga
      361 gaacctctac ttccaatcgc accatcatca ccaccatgat tacaaggatg acgacgataa
      421 gtgaggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
      481 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      541 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      601 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      841 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgcccctga tagacggttt
      901 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgtt
//
