LOCUS       sequence-52987-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD2
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             744..752
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             811..831
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             832..849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             850..873
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attttgttta ccttaagaag gagatatact atggcagcat gccgtgcatt aaaagctgtt
       61 ttggtagatc tcagtggcac acttcacatt gaagatgcag ctgtgccagg cgcacaggaa
      121 gctcttaaaa ggttacgtgg tgcttctgta atcattaggt ttgtgaccaa tacaaccaaa
      181 gagagcaagc aagacctgtt agaaaggttg agaaaattgg aatttgatat ctctgaagat
      241 gaaatattca catctctgac tgcagccaga agtttactag agcggaaaca agtcagaccc
      301 atgctgctag ttgatgatcg ggcactacct gatttcaaag gaatacaaac aagtgatcct
      361 aatgctgtgg tcatgggatt ggcaccagaa cattttcatt atcaaattct gaatcaagca
      421 ttccggttac tcctggatgg agcacctctg atagcaatcc acaaagccag gtattacaag
      481 aggaaagatg gcttagccct ggggcctgga ccatttgtga ctgctttaga gtatgccaca
      541 gataccaaag ccacagtcgt ggggaaacca gagaagacgt tctttttgga agcattgcgg
      601 ggcactggct gtgaacctga ggaggctgtc atgataggag atgattgcag ggatgatgtt
      661 ggtggggctc aagatgtcgg catgctgggc atcttagtaa agactgggaa atatcgagca
      721 tcagatgaag aaaaaattaa tccacctcct tacttaactt gtgagagttt ccctcatgct
      781 gtggaccaca ttctgcagca cctattggca gagaacctct acttccaatc gcaccatcat
      841 caccaccatg attacaagga tgacgacgat aagtgaggat ccgaattcga gctccgtcga
      901 caagcttgcg ggccgcact
//
