LOCUS       sequence-52988-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD9
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             529..546
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(599..617)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      613..660
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(784..803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg ctgaaactga gcgcggaaaa
      121 tgaaagcacc ccgattcagc agctgctgga acatttcctg cgccagctgc agcgtaaaga
      181 tccgcatggc ttttttgcgt tcccggtgac cgatgcgatt gcgccgggtt atagcatgat
      241 tattaaacat ccgatggatt ttggtaccat gaaagataaa atcgtggcga atgaatataa
      301 aagcgtgacc gaatttaaag ccgatttcaa actgatgtgc gataacgcga tgacctataa
      361 ccgtccggat accgtgtatt ataaactggc caaaaaaatt ctgcatgcgg gttttaaaat
      421 gatgagcaaa gaacgtctgc tggccctgaa acgcagcatg agctgacagt aaaggtggat
      481 acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      541 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      601 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      661 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      841 ctctaaatcg ggggctcccc tttaggttcc gatttagtgc tttacggcac ctcgacccca
      901 aaaaacttga ttagggtga
//
