LOCUS       sequence-52989-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TAF1L
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             566..583
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(636..654)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      650..697
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(821..840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt
       61 ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgcaggtg gcctttagct
      121 ttatcctgga taatatcgtg acccagaaaa tgatggcggt gccggatagc tggccgtttc
      181 atcatccggt taacaaaaaa tttgttccgg attattataa aatgattgtg aatccggttg
      241 atctggaaac catccgtaaa aatattagca aacataaata tcagagccgc gaaagctttc
      301 tggatgatgt gaacctgatt ctggccaaca gcgttaaata taacggtccg gaaagccagt
      361 ataccaaaac cgcgcaggaa attgtgaata tttgctatca gaccattacc gaatatgatg
      421 aacatctgac ccagctggaa aaagatattt gcaccgcgaa agaagcggcg ctggaagaag
      481 ccgaactgga aagcctggat tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
      541 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      601 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      661 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      721 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      781 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      841 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      901 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      961 tcacgt
//
