LOCUS       sequence-52990-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTS
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             574..591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(644..662)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      658..705
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(829..848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggccgtcgc tgccaggcac aagtgtcccg
      121 ccgcatctcc ttcagcgcga gccaccgatt gtacagtaaa tttctaagtg atgaagaaaa
      181 cttgaaactg tttgggaaat gcaacaatcc aaatggccat gggcacaatt ataaagttgt
      241 ggtgacagta catggagaga ttgaccctgc tacgggaatg gttatgaatc tggctgatct
      301 caaaaaatat atggaggagg cgattatgca gccccttgat cataagaatc tggatatgga
      361 tgtgccatac tttgcagatg tggtgagcac gactgaaaat gtagctgttt atatctggga
      421 caacctccag aaagttcttc ctgtaggagt tctttataaa gtaaaagtat acgaaactga
      481 caataatatt gtggtttata aaggagaatg acagtaaagg tggatacgga tccgaattcg
      541 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      601 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      661 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      721 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      901 tccctttagg gttccgattt agtgctttac ggc
//
