LOCUS       sequence-52994-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MDP1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..79
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             640..657
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(710..728)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      724..771
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aggagatata catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga
       61 gaacctgtac ttccaatcca tggcgcggct accgaagctg gcagtctttg atttggatta
      121 cactctctgg cctttctggg tcgacacgca cgtagaccct ccgttccata agagcagtga
      181 tggaactgta cgagataggc ggggccaaga cgtccgactg tacccagagg tgcctgaggt
      241 cctaaaacga ttgcagagcc ttggggtgcc cggtgcggct gcttcaagga caagtgagat
      301 agaaggggcc aaccagctac tggagctctt tgacctcttc aggtactttg ttcatcggga
      361 aatctatcca ggcagcaaga tcacacactt tgagaggttg cagcagaaga ctggaattcc
      421 tttctcccag atgatcttct ttgatgatga gaggcggaat attgtagacg tcagcaaact
      481 gggtgttacc tgcattcaca tccagaatgg aatgaatctt caaactctaa gtcaagggtt
      541 agagacattt gcgaaggccc aaactgggcc tttgtgacag taaaggtgga tacggatccg
      601 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      661 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      721 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      781 ggaactatat ccggattggc gaatgggacg cgccctgta
//
