LOCUS       sequence-53001-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: EP300
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..44
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             479..496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(549..567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      563..610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggcg ccgggccaga
       61 gcaaaaaaaa aattttcaaa ccggaagaac tgcgtcaggc cctgatgccg accctggaag
      121 ccctgtatcg tcaggatccg gaaagcctgc cgtttcgcca gccggtggat ccgcagctgc
      181 tgggtatccc ggattatttt gatattgtta aaagcccgat ggatctgagc accattaaac
      241 gtaaactgga taccggccag tatcaggaac cgtggcagta tgttgatgat atttggctga
      301 tgtttaataa tgcgtggctg tataatcgca aaaccagccg cgtttataaa tattgtagca
      361 aactgagcga agtgtttgaa caggaaattg atccggtgat gcagagcctg ggctgacagt
      421 aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      481 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      601 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
//
