LOCUS       sequence-53009-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N
  TITLE     Abr and Bcr, two homologous Rac GTPase-activating proteins, control 
            multiple cellular functions of murine macrophages.
  JOURNAL   Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.
  PUBMED    17116687
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2007-02"; volume: "27(3)"; pages: "899-911. Epub 2006
            Nov 20"
COMMENT     Sequence Label: pCDNA3.1Xpress-BcrGAP R1090A
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             119..136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..172
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(176..184,185..199)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttaccatgg ggggttctca
      121 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      181 gtacgacgat gacgataagg tacctaggat ccccctggac ccgcaggccc tgcaggacag
      241 agactggcag cgcaccgtca tcgccatgaa tgggatcgaa gtaaagctct cggtcaagtt
      301 caacagcagg gagttcagct tgaagaggat gccgtcccga aaacagacag gggtcttcgg
      361 agtcaagatt gctgtggtca ccaagagaga gaggtccaag gtgccctaca tcgtgcgcca
      421 gtgcgtggag gagatcgagc gccgaggcat ggaggaggtg ggcatctacg ccgtgtccgg
      481 tgtggccacg gacatccagg cactgaaggc agccttcgac gtcaataaca aggatgtgtc
      541 ggtgatgatg agcgagatgg acgtgaacgc catcgcaggc acgctgaagc tgtacttccg
      601 tgagctgccc gagcccctct tcactgacga gttctacccc aacttcgcag agggcatcgc
      661 tctttcagac ccggttgcaa aggagagctg catgctcaac ctgctgctgt ccctgccgga
      721 ggccaacctg ctcaccttcc ttttccttct ggaccacctg aaaagggtgg cagagaagga
      781 ggcagtcaat aagatgtccc tgcacaacct cgccacggtc tttggcc
//
